Grace, C.E.E., Lakshmi, P.K., Meenakshi, S. et al. (3 more authors) (2020) Biomolecular transitions and lipid accumulation in green microalgae monitored by FTIR and Raman analysis. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 224. 117382. ISSN 1386-1425
Abstract
Fourier transform infrared (FTIR) and Raman spectroscopic techniques were employed to analyze the biomolecular transitions and lipid accumulation in three freshwater green microalgal species, Monoraphidium contortum (M. contortum), Pseudomuriella sp. and Chlamydomonas sp. during various phases of their growth. Biomolecular transitions and lipid [hydrocarbons, triacylglycerides (TAGs)] accumulation within the microalgal cells were identified using second derivatives of the FTIR absorption spectroscopy. Second derivative analysis normalized and resolved the original spectra and led to the identification of smaller, overlapping bands. Both relative and absolute content of lipids were determined using the integrated band area. M. contortum exhibited higher accumulation of lipids than the other two species. The integrated band area of the vibrations from saturated (SFA) and unsaturated lipids (UFA) enabled quantification of fatty acids. The percentage of SFA and UFA was determined using GC, FTIR and Raman spectroscopy. From the spectral data, the order of increasing concentration of SFA among the three microalgal species was M. contortum > Chlamydomonas sp. > Pseudomuriella sp. The spectral results on fatty acids were consistent with the separation of lipids by gas chromatography. The results emphasized the significance of FTIR and Raman spectroscopic methods in monitoring the biomolecular transitions and rapid quantification of lipids, without the need for extraction of lipids.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2019 Elsevier. This is an author produced version of a paper subsequently published in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. Uploaded in accordance with the publisher's self-archiving policy. Article available under the terms of the CC-BY-NC-ND licence (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
Keywords: | Vibrational spectroscopy; Infrared; Hydrocarbons; Lipid; Biodiesel; Biofuel; Bioenergy |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Chemical and Biological Engineering (Sheffield) |
Funding Information: | Funder Grant number Biotechnology and Biological Sciences Research Council (BBSRC) BB/K020633/1 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 29 Jul 2019 10:30 |
Last Modified: | 14 Jul 2020 00:38 |
Status: | Published |
Publisher: | Elsevier |
Refereed: | Yes |
Identification Number: | 10.1016/j.saa.2019.117382 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:149021 |
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