Chávez, JC, Vicens, A, Wrighton, DC et al. (5 more authors) (2019) A cytoplasmic Slo3 isoform is expressed in somatic tissues. Molecular Biology Reports, 46 (5). pp. 5561-5567. ISSN 0301-4851
Abstract
Slo3 is a pH-sensitive and weakly voltage-sensitive potassium channel that is essential for male fertility in mouse and whose expression is regarded as sperm-specific. These properties have proposed Slo3 as a candidate target for male contraceptive drugs. Nonetheless, the tissue distribution of Slo3 expression has not been rigorously studied yet. Applying computational and RT-PCR approaches, we identified expression of two short Slo3 isoforms in somatic mouse tissues such as brain, kidney and eye. These isoforms, which seem to result of transcription starting sites between exons 20 and 21, have an identical open reading frame, both encoding the terminal 381 amino acids of the cytosolic Slo3 domain. We corroborated the expression of these isoforms in mouse brain and testis by Western-blot. The complete isoform encoding the Slo3 ion channel was uniquely detected in testis, both at transcript and protein level. Although the functional role of the cytosolic Slo3 isoforms remains to be established, we propose that they may have a functional effect by modulating Slo channels trafficking and/or activity. This study confirms that expression of full-length Slo3 is sperm-specific but warns against developing contraceptive drugs targeting the C-terminal tail of Slo3 channels.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © Springer Nature B.V. 2019. This is a post-peer-review, pre-copyedit version of an article published in Molecular Biology Reports. The final authenticated version is available online at: https://doi.org/10.1007/s11033-019-04943-z |
Keywords: | Potassium channels; Slo; Alternative splicing; Sperm |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biomedical Sciences (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 11 Jul 2019 09:52 |
Last Modified: | 03 Jul 2020 00:40 |
Status: | Published |
Publisher: | Springer |
Identification Number: | 10.1007/s11033-019-04943-z |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:148447 |