Wang, S, Redmile-Gordon, M, Mortimer, M et al. (5 more authors) (2019) Extraction of extracellular polymeric substances (EPS) from red soils (Ultisols). Soil Biology and Biochemistry, 135. pp. 283-285. ISSN 0038-0717
Abstract
Extracellular polymeric substances (EPS) have many beneficial functions in soils. Accurate quantification of EPS in soils is crucial. Here, five methods were compared for their suitability for extraction of EPS from Ultisols: hot water extractable polysaccharide (HWEP), hot dilute acid extractable polysaccharide (HDAEP), easily extractable glomalin (EEG), sodium sulfide (SS) and cation exchange resin (CER) method. Humic-acid equivalent (HAE) was used as an indicator for extracellular contamination and ATP for quantifying intracellular contamination from cell lysis. Among the tested methods, CER resulted in EPS extraction with minimal contamination. Therefore, we propose that CER is currently the most appropriate method for extraction of EPS from Ultisols.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2019 Elsevier Ltd. This is an author produced version of a paper published in Soil Biology and Biochemistry. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Soil biofilms; Extracellular polymeric substances; Polysaccharide; Cation exchange resin; Easily extractable glomalin |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Environment (Leeds) > School of Earth and Environment (Leeds) > Earth Surface Science Institute (ESSI) (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 06 Jun 2019 10:52 |
Last Modified: | 20 May 2020 00:38 |
Status: | Published |
Publisher: | Elsevier Ltd. |
Identification Number: | 10.1016/j.soilbio.2019.05.014 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:146966 |
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