Patil, S, Babu, N, Subbannayya, T et al. (11 more authors) (2019) Secretome analysis of oral keratinocytes chronically exposed to shisha. Cancer Biomarkers, 25 (1). pp. 29-41. ISSN 1574-0153
Abstract
BACKGROUND:
Shisha smoking has been associated with multiple diseases including oral cancer. However, a mechanistic study to investigate alteration of secreted proteins in oral cells due to shisha smoking is lacking.
OBJECTIVES:
Elucidation of differentially secreted proteins by immortalized human normal oral keratinocytes (OKF6/TERT1) upon chronic exposure to shisha.
METHODS:
OKF6/TERT1 was chronically treated with 0.5% shisha extract for 8 months. Conditioned media from shisha treated (OKF6/TERT1-Shisha) and untreated (OKF6/TERT1-Parental) cells were subjected to TMT-based quantitative proteomic analysis. Bioinformatics analysis of differentially secreted proteins was carried out using SignalP, SecretomeP and TMHMM. Immunoblot validation of selected proteins was carried out to confirm the proteomics results.
RESULTS:
Proteomic analysis of OKF6/TERT1-Parental and OKF6/TERT1-Shisha secretome resulted in the identification of 1,598 proteins, of which 218 proteins were found to be differentially secreted (⩾ 1.5-fold; p-value ⩽ 0.05) in shisha treated cells. Bioinformatics analysis using prediction tools showed secretory potential of differentially secreted proteins identified in OKF6/TERT1-Shisha. Western blotting validated the expression of AKR1C2, HSPH1 and MMP9 in OKF6/TERT1-Shisha secretome in agreement with proteomic data.
CONCLUSION:
This study serves as a useful resource to understand the effect of chronic shisha smoking on the milieu of secreted proteins of oral cells. In vivo studies are warranted to supplement our in vitro data to elucidate the role of these proteins as early diagnostic biomarkers for oral carcinogenesis among shisha smokers.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | This paper is protected by copyright. This is an author produced version of a paper published in Cancer Biomarkers. The final publication is available at IOS Press through https://doi.org/10.3233/CBM-182099. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | OKF6/TERT1; secretory biomarkers; waterpipe; TMT; oral cancer; proteomics |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Dentistry (Leeds) > Restorative Dentistry (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 09 Apr 2019 11:00 |
Last Modified: | 12 Jun 2019 18:23 |
Status: | Published |
Publisher: | IOS Press |
Identification Number: | 10.3233/CBM-182099 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:144686 |