Hawkins, Michelle Sarah orcid.org/0000-0001-9393-8206, Dimude, Juachi U., Howard, Jamieson Anthony Leyland orcid.org/0000-0002-4694-5427 et al. (5 more authors) (2019) Direct removal of RNA polymerase barriers to replication by accessory replicative helicases. Nucleic Acids Research. pp. 5100-5113. ISSN 0305-1048
Abstract
Bacterial genome duplication and transcription require simultaneous access to the same DNA template. Conflicts between the replisome and transcription machinery can lead to interruption of DNA replication and loss of genome stability. Pausing, stalling and backtracking of transcribing RNA polymerases add to this problem and present barriers to replisomes. Accessory helicases promote fork movement through nucleoprotein barriers and exist in viruses, bacteria and eukaryotes. Here, we show that stalled Escherichia coli transcription elongation complexes block reconstituted replisomes. This physiologically relevant block can be alleviated by the accessory helicase Rep or UvrD, resulting in the formation of full-length replication products. Accessory helicase action during replication-transcription collisions therefore promotes continued replication without leaving gaps in the DNA. In contrast, DinG does not promote replisome movement through stalled transcription complexes in vitro. However, our data demonstrate that DinG operates indirectly in vivo to reduce conflicts between replication and transcription. These results suggest that Rep and UvrD helicases operate on DNA at the replication fork whereas DinG helicase acts via a different mechanism.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) The University of York > Faculty of Sciences (York) > Physics (York) |
Depositing User: | Pure (York) |
Date Deposited: | 27 Feb 2019 10:30 |
Last Modified: | 23 Jan 2025 00:18 |
Published Version: | https://doi.org/10.1093/nar/gkz170 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1093/nar/gkz170 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:143043 |
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