Harton, Kaja and Shimizu, Seishi orcid.org/0000-0002-7853-1683 (2019) Statistical thermodynamics of casein aggregation::Effects of salts and water. Biophysical Chemistry. pp. 34-42. ISSN 0301-4622
Abstract
Salts, when added to milk, profoundly influence casein aggregation.. Even though this well-known phenomenon has been widely exploited, there are still many unanswered questions. How do salts affect casein aggregation? Does water contribute significantly to the aggregation change? The key to answering these questions comes from statistical thermodynamics, i.e., the principles of physics that can link macroscopic data to the collective behaviour of molecules. We present two theoretical approaches, a rigorous approach which demands far more measurements than reported hitherto, and an approximate and pragmatic approach based on stoichiometric models (isodesmic model for aggregation equilibria and von Smoluchowski model for kinetics) that can yield information on protein-water and protein-salt interactions from “real life” experimental measurements on a model systems available in a wide variety of formats. Using the experimental data in the literature, casein aggregation in the absence of κ-casein has been shown to be modulated by protein-salt interaction, while the contribution from water structure changes has been shown to be negligible.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2019 Elsevier B.V. All rights reserved. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) |
Depositing User: | Pure (York) |
Date Deposited: | 13 Feb 2019 11:30 |
Last Modified: | 16 Oct 2024 14:45 |
Published Version: | https://doi.org/10.1016/j.bpc.2019.02.004 |
Status: | Published online |
Refereed: | Yes |
Identification Number: | 10.1016/j.bpc.2019.02.004 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:142433 |
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Filename: Casein_Biophysical_Chemistry_Revised.docx
Description: Casein Biophysical Chemistry Revised
Licence: CC-BY-NC-ND 2.5