Liang, H, Russell, SJ orcid.org/0000-0003-0339-9611, Wood, DJ orcid.org/0000-0001-8269-9123 et al. (1 more author) (2018) Monomer-induced customisation of UV-cured atelocollagen hydrogel networks. Frontiers in Chemistry, 6. 626. ISSN 2296-2646
Abstract
The covalent functionalisation of type I atelocollagen with either 4 vinylbenzyl or methacrylamide residues is presented as a simple synthetic strategy to achieve customisable, cell-friendly UV cured hydrogel networks with widespread clinical applicability. Molecular parameters, i.e. the type of monomer, degree of atelocollagen functionalisation and UV curing solution, have been systematically varied and their effect on gelation kinetics, swelling behaviour, elastic properties and enzymatic degradability investigated. UV-cured hydrogel networks deriving from atelocollagen precursors functionalised with equivalent molar content of 4 vinylbenzyl (F4VBC= 18±1 mol.%) and methacrylamide (FMA= 19±2 mol.%) adducts proved to display remarkably-different swelling ratio (SR= 1963±58–5202±401 wt.%), storage modulus (G’= 17±3–390±99 Pa) and collagenase resistance (µrel= 18±5–56±5 wt.%), similarly to the case of UV cured hydrogel networks obtained with the same type of methacrylamide adduct, but varied degree of functionalisation (FMA= 19±2–88±1 mol.%). UV-induced network formation of 4VBC functionalised atelocollagen molecules yielded hydrogels with increased stiffness and enzymatic stability, attributed to the molecular rigidity of resulting aromatised crosslinking segment, whilst no toxic response was observed with osteosarcoma G292 cells. Although to a lesser extent, the pH of the UV-curing solution also proved to affect macroscopic hydrogel properties, likely due to the altered organisation of atelocollagen molecules during network formation. By leveraging the knowledge gained with classic synthetic networks, this study highlights how the type of monomer can be conveniently exploited to realise customisable atelocollagen hydrogels with controlled structure-property relationships to meet the requirements of unmet clinical applications.
Metadata
Item Type: | Article |
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Copyright, Publisher and Additional Information: | © 2018 Liang, Russell, Wood and Tronci. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
Keywords: | Type I atelocollagen; Covalent network; UV-curing; monomer; 4-Vinylbenzyl chloride; Methacrylic anhydride |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Arts, Humanities and Cultures (Leeds) > School of Design (Leeds) The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Dentistry (Leeds) > Oral Biology (Leeds) |
Funding Information: | Funder Grant number EPSRC EP/K029592/1 EPSRC EP/R511717/1 |
Depositing User: | Symplectic Publications |
Date Deposited: | 04 Dec 2018 12:07 |
Last Modified: | 05 Feb 2019 13:52 |
Status: | Published |
Publisher: | Frontiers Media |
Identification Number: | 10.3389/fchem.2018.00626 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:139497 |