Majiya, H, Adeyemi, OO orcid.org/0000-0002-0848-5917, Herod, M et al. (2 more authors) (2018) Photodynamic inactivation of non-enveloped RNA viruses. Journal of Photochemistry and Photobiology B: Biology, 189. pp. 87-94. ISSN 1011-1344
Abstract
We recently reported the photodynamic inactivation (PDI) of bacteriophage MS2 with a photosensitiser- 5, 10, 15, 20-tetrakis (1-methyl-4-pyridinio) porphyrin- tetra- p-toluene sulfonate (TMPyP) in solution and concluded that the A-protein of the virus is the main target of inactivation. Here, we have extended these studies and carried out PDI of bacteriophage Qβ, bovine enterovirus 2 (BEV-2) and type 1 murine norovirus (MNV-1). The rate of inactivation observed was in the order MS2 > Qβ > MNV-1 > BEV-2. Data suggested that TMPyP-treatment could also target the viral genome as well as result in disintegration/disassembly of viral particles. Although emergence of viral drug resistance is a well-documented phenomenon, it was not possible to generate PDI-resistant MS2. However, emergence of a mutation in the lysis protein was detected after serial exposure to PDI.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2018 Elsevier B.V. This is an author produced version of a paper published in Journal of Photochemistry and Photobiology B: Biology. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | A-protein; MS2; Bovine enterovirus; Murine norovirus; Singlet oxygen; TMPyP; Photodynamic inactivation |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biomedical Sciences (Leeds) The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Molecular Virology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 17 Oct 2018 10:09 |
Last Modified: | 09 Oct 2019 00:38 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.jphotobiol.2018.10.009 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:137176 |
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