Dalton, Charlotte E., Quinn, Steven D. orcid.org/0000-0003-3442-4103, Rafferty, Aidan et al. (3 more authors) (2016) Single-Molecule Fluorescence Detection of a Synthetic Heparan Sulfate Disaccharide. ChemPhysChem. pp. 3442-3446. ISSN 1439-4235
Abstract
The first single-molecule fluorescence detection of a structurally-defined synthetic carbohydrate is reported: a heparan sulfate (HS) disaccharide fragment labeled with Alexa488. Single molecules have been measured whilst freely diffusing in solution and controlled encapsulation in surface-tethered lipid vesicles has allowed extended observations of carbohydrate molecules down to the single-molecule level. The diverse and dynamic nature of HS–protein interactions means that new tools to investigate pure HS fragments at the molecular level would significantly enhance our understanding of HS. This work is a proof-of-principle demonstration of the feasibility of single-molecule studies of synthetic carbohydrates which offers a new approach to the study of pure glycosaminoglycan (GAG) fragments.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | ©2016 The Authors. |
Keywords: | carbohydrate,fluorescence,heparan sulfate,single-molecule studies,vesicle |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Physics (York) The University of York > Faculty of Sciences (York) > Biology (York) |
Depositing User: | Pure (York) |
Date Deposited: | 21 Jun 2018 10:20 |
Last Modified: | 12 Dec 2024 00:12 |
Published Version: | https://doi.org/10.1002/cphc.201600750 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1002/cphc.201600750 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:132415 |
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