Bawazer, LA, Ihli, J, Levenstein, MA orcid.org/0000-0002-2309-3743 et al. (3 more authors) (2018) Enzymatically-Controlled Biomimetic Synthesis of Titania/Protein Hybrid Thin films. Journal of Materials Chemistry B, 6. pp. 3979-3988. ISSN 2050-750X
Abstract
Although it is widely recognised that enzymes play a significant role in sculpting complex silica skeletons in marine sponges, the potential for exploiting enzymes in materials synthesis has not yet been fully harnessed. In this work we show that the digestive enzyme papain can self-assemble into monolayers on oxide surfaces, where they then drive the formation of crystalline titanium dioxide nanoparticles. This dual functionality of thin film formation and mineralization promotion has the potential to enable the construction of hierarchical inorganic/organic structures in the form of continuous amorphous titania/protein films which can be refined to 93% anatase post annealing. Additional control over the film thickness is afforded by layer-by-layer processing using a simple dip-coating approach. Papain's TiO₂-mineralizing activity displays complex kinetics that deviates from the native Michaelis–Menten kinetic activity, yet deactivation studies demonstrate that this activity relies upon residues that are essential for catalytic site function. These parameters provide unique insight into enzymatic biomineralization, allowing a flexible route to achieving bioengineered titania heterostructures, and potentially providing a green-chemistry solution to photovoltaic cell development.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © The Royal Society of Chemistry 2018. This is an author produced version of a paper published in Journal of Materials Chemistry B . Uploaded in accordance with the publisher's self-archiving policy. |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biomedical Sciences (Leeds) The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Mechanical Engineering (Leeds) The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) > Inorganic Chemistry (Leeds) |
Funding Information: | Funder Grant number EPSRC EP/H005374/1 |
Depositing User: | Symplectic Publications |
Date Deposited: | 25 May 2018 11:22 |
Last Modified: | 23 May 2019 00:42 |
Status: | Published |
Publisher: | Royal Society of Chemistry |
Identification Number: | 10.1039/C8TB00381E |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:131298 |