Chapman, MR, Cosgrove, SC, Turner, NJ et al. (2 more authors) (2018) Highly Productive Oxidative Biocatalysis in Continuous Flow by Enhancing the Aqueous Equilibrium Solubility of Oxygen. Angewandte Chemie - International Edition, 57 (33). pp. 10535-10539. ISSN 1433-7851
Abstract
We report a simple, mild and synthetically clean approach to accelerate the rate of enzymatic oxidation reactions by up to a factor of 100 when compared to conventional batch gas/liquid systems. Biocatalytic decomposition of H2O2 is used to produce a soluble source of O2 directly in reaction media, enabling the ambient solubility of aqueous O2 to be increased under safe and practical conditions. To best exploit the method, a novel flow reactor was developed to maximize productivity (g product/L/h). This scalable benchtop protocol provides a distinct advantage over conventional bio‐oxidation, in that no pressurized gas or specialist equipment is employed. The method is general across different oxidase enzymes and compatible with a variety of functional groups. These results culminate in record space‐time‐yields for bio‐oxidation.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2018 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is the peer reviewed version of the following article: Chapman, M. ., Cosgrove, S. ., Turner, N. ., Kapur, N. and Blacker, A. J. (2018), Highly Productive Oxidative Biocatalysis in Continuous‐Flow by Surpassing the Aqueous Equilibrium Solubility of Oxygen. Angew. Chem. Int. Ed. doi:10.1002/anie.201803675, which has been published in final form at https://doi.org/10.1002/anie.201803675. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | bio-oxidation; biocatalysis; continuous flow; flow reactors; oxidation; space-time yields |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) > Organic Chemistry (Leeds) The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Mechanical Engineering (Leeds) > Institute of Engineering Thermofluids, Surfaces & Interfaces (iETSI) (Leeds) |
Funding Information: | Funder Grant number BBSRC (Biotechnology & Biological Sciences Research Council) BIOCATNET PoC-013 HEFCE (Higher Education Funding Council for England) no ext ref ACS Green Chemistry Institute Not Known EPSRC (Engineering and Physical Sciences Research Council) EP/R511717/1 |
Depositing User: | Symplectic Publications |
Date Deposited: | 16 May 2018 13:54 |
Last Modified: | 03 Apr 2020 02:55 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1002/anie.201803675 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:130668 |