Di Silvio, D, Maccarini, M, Parker, R et al. (3 more authors) (2017) The effect of the protein corona on the interaction between nanoparticles and lipid bilayers. Journal of Colloid and Interface Science, 504. pp. 741-750. ISSN 0021-9797
Abstract
Hypothesis It is known that nanoparticles (NPs) in a biological fluid are immediately coated by a protein corona (PC), composed of a hard (strongly bounded) and a soft (loosely associated) layers, which represents the real nano-interface interacting with the cellular membrane in vivo. In this regard, supported lipid bilayers (SLB) have extensively been used as relevant model systems for elucidating the interaction between biomembranes and NPs. Herein we show how the presence of a PC on the NP surface changes the interaction between NPs and lipid bilayers with particular care on the effects induced by the NPs on the bilayer structure. Experiments In the present work we combined Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D) and Neutron Reflectometry (NR) experimental techniques to elucidate how the NP-membrane interaction is modulated by the presence of proteins in the environment and their effect on the lipid bilayer. Findings Our study showed that the NP-membrane interaction is significantly affected by the presence of proteins and in particular we observed an important role of the soft corona in this phenomenon.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017 Elsevier Inc. All rights reserved. This is an author produced version of a paper published in Journal of Colloid and Interface Science. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Supported lipid bilayer; Protein corona nanoparticles; Quartz crystal microbalance |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) |
Funding Information: | Funder Grant number BBSRC BB/P023827/1 |
Depositing User: | Symplectic Publications |
Date Deposited: | 29 Jan 2018 17:00 |
Last Modified: | 29 May 2018 00:38 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.jcis.2017.05.086 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:126698 |