Smith, CEL orcid.org/0000-0001-8320-5105, Alexandraki, A, Cordery, SF et al. (3 more authors) (2017) A tissue-specific promoter derived from a SINE retrotransposon drives biallelic expression of PLAGL1 in human lymphocytes. PLoS ONE, 12 (9). e0185678. ISSN 1932-6203
Abstract
The imprinted gene PLAGL1 is an important regulator of apoptosis and cell cycle arrest. Loss of its expression has been implicated in tumorigenesis in a range of different cancers, and overexpression during fetal development causes transient neonatal diabetes mellitus (TNDM). PLAGL1 lies within an imprinted region of chromosome 6q24, and monoallelic expression from the major, differentially methylated promoter (P1) occurs in most human tissues. However, in peripheral blood leukocytes, the active promoter (P2) is non-imprinted and drives biallelic transcription. We report here a novel PLAGL1 promoter (P5) derived from the insertion of a primate-specific, MIR3 SINE retrotransposon. P5 is highly utilized in lymphocytes, particularly in T cells, and like P2, directs biallelic transcription. Our results show that it is important to consider P5 in relation to PLAGL1 function in T cells when investigating the dysregulation of this gene.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | (c) 2017 Smith et al. This is an open access article distributed under the terms of the Creative Commons Attribution License [https://creativecommons.org/licenses/by/4.0/], which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
Keywords: | genomic imprinting; DNA methylation; tumour suppressor; promoter; T lymphocytes; SINE retrotransposon |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Inst of Biomed & Clin Sciences (LIBACS) (Leeds) > Genetics (LIBACS) (Leeds) |
Funding Information: | Funder Grant number Yorkshire Cancer Research CHARACTERISATION OF A... Expired: Use BreastCancerNow 605321 NOT GIVEN Yorkshire Cancer Research L330 Yorkshire Cancer Research Not Known |
Depositing User: | Symplectic Publications |
Date Deposited: | 02 Oct 2017 14:53 |
Last Modified: | 23 Jun 2023 22:36 |
Status: | Published |
Publisher: | Public Library of Science |
Identification Number: | 10.1371/journal.pone.0185678 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:121843 |