Paterson, T.E., Beal, S.N., Santocildes-Romero, M.E. et al. (3 more authors) (2017) Selective laser melting–enabled electrospinning: Introducing complexity within electrospun membranes. Proceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine, 231 (6). pp. 565-574. ISSN 0954-4119
Abstract
Additive manufacturing technologies enable the creation of very precise and well-defined structures that can mimic hierarchical features of natural tissues. In this article, we describe the development of a manufacturing technology platform to produce innovative biodegradable membranes that are enhanced with controlled microenvironments produced via a combination of selective laser melting techniques and conventional electrospinning. This work underpins the manufacture of a new generation of biomaterial devices that have significant potential for use as both basic research tools and components of therapeutic implants. The membranes were successfully manufactured and a total of three microenvironment designs (niches) were chosen for thorough characterisation. Scanning electron microscopy analysis demonstrated differences in fibre diameters within different areas of the niche structures as well as differences in fibre density. We also showed the potential of using the microfabricated membranes for supporting mesenchymal stromal cell culture and proliferation. We demonstrated that mesenchymal stromal cells grow and populate the membranes penetrating within the niche-like structures. These findings demonstrate the creation of a very versatile tool that can be used in a variety of tissue regeneration applications including bone healing.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017 SAGE Publications. This is an author produced version of a paper subsequently published in Proceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Additive manufacturing; selective laser melting; electrospinning; stem cell niche; bone healing |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > School of Clinical Dentistry (Sheffield) |
Funding Information: | Funder Grant number ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL (EPSRC) EP/K029592/1 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 08 Sep 2017 14:45 |
Last Modified: | 09 Sep 2017 01:50 |
Published Version: | https://doi.org/10.1177/0954411917690182 |
Status: | Published |
Publisher: | SAGE Publications |
Refereed: | Yes |
Identification Number: | 10.1177/0954411917690182 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:120988 |