Bedford, R, Tiede, C orcid.org/0000-0003-0280-4005, Hughes, R et al. (4 more authors) (2017) Alternative reagents to antibodies in imaging applications. In: Biophysical Reviews. 19th IUPAB Congress, 16-20 Jul 2017, Edinburgh, UK. Springer , pp. 299-308.
Abstract
Antibodies have been indispensable tools in molecular biology, biochemistry and medical research. However, a number of issues surrounding validation, specificity and batch variation of commercially available antibodies have prompted research groups to develop novel non-antibody binding reagents. The ability to select highly specific monoclonal non-antibody binding proteins without the need for animals, the ease of production and the ability to site-directly label has enabled a wide variety of applications to be tested, including imaging. In this review, we discuss the success of a number of non-antibody reagents in imaging applications, including the recently reported Affimer.
Metadata
Item Type: | Proceedings Paper |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © The Author(s) 2017. Open Access: This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
Keywords: | Affimer; Adhiron; DARPin; Monobody; Imaging; Super-resolution |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Cell Biology (Leeds) The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Synthetic Biology (Leed) |
Depositing User: | Symplectic Publications |
Date Deposited: | 08 Aug 2017 10:25 |
Last Modified: | 23 Jun 2023 22:33 |
Status: | Published |
Publisher: | Springer |
Identification Number: | 10.1007/s12551-017-0278-2 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:119868 |