Shimizu, Seishi orcid.org/0000-0002-7853-1683 and Matubayasi, Nobuyuki (2017) Osmolyte depletion viewed in terms of the dividing membrane and its work of expansion against osmotic pressure. Biophysical Chemistry. pp. 111-115. ISSN 0301-4622
Abstract
How osmolytes enhance the folding, binding, and self-assembly of biological macromolecules at a microscopic scale has long been a matter of debate. Ambiguities persist on the key interpretive concepts, such as the “effective membrane” (which marks the boundary of the volume from which osmolytes are excluded) and the “free energy of exclusion” of osmolytes from biomolecular surfaces. In this paper, we formulate these elusive concepts based upon chemical thermodynamics and rigorous statistical thermodynamics (the Kirkwood-Buff theory). Positioning of the membrane at the osmotic dividing surface is crucial in order not to affect the thermodynamics of solvation. The notion of the free energy (work) of excluding osmolytes is refined to the expansion work against the osmotic pressure, which indeed describes the change of solvation free energy at dilute osmolyte concentrations.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017 Elsevier B.V. All rights reserved. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details. |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) |
Depositing User: | Pure (York) |
Date Deposited: | 07 Mar 2017 15:20 |
Last Modified: | 23 Oct 2024 00:02 |
Published Version: | https://doi.org/10.1016/j.bpc.2017.02.003 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1016/j.bpc.2017.02.003 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:113325 |
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