Lam, LQ, Wong, BX, Frugier, T et al. (5 more authors) (2017) Oxidation of Iron under Physiologically Relevant Conditions in Biological Fluids from Healthy and Alzheimer's Disease Subjects. ACS Chemical Neuroscience, 8 (4). pp. 731-736. ISSN 1948-7193
Abstract
Ferroxidase activity has been reported to be altered in various biological fluids in neurodegenerative disease, but the sources contributing to the altered activity are uncertain. Here we assay fractions of serum and cerebrospinal fluid with a newly validated triplex ferroxidase assay. Our data indicate that while ceruloplasmin, a multicopper ferroxidase, is the predominant source of serum activity, activity in CSF predominantly derives from a <10 kDa component, specifically from polyanions such as citrate and phosphate. We confirm that in human biological samples, ceruloplasmin activity in serum is decreased in Alzheimer's disease, but in CSF a reduction of activity in Alzheimer's disease originates from the polyanion component.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017, American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Chemical Neuroscience, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [insert ACS Articles on Request author-directed link to Published Work, see https://doi.org/10.1021/acschemneuro.6b00411. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | ceruloplasmin; Ferroxidase; iron; neurodegenerative disease; oxidation; polyanion |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biomedical Sciences (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 21 Feb 2017 10:03 |
Last Modified: | 28 Dec 2017 01:38 |
Published Version: | https://doi.org/10.1021/acschemneuro.6b00411 |
Status: | Published |
Publisher: | American Chemical Society |
Identification Number: | 10.1021/acschemneuro.6b00411 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:112605 |