Tyler, AII orcid.org/0000-0003-2116-1084, Barriga, HMG, Parsons, ES et al. (5 more authors) (2015) Electrostatic swelling of bicontinuous cubic lipid phases. Soft Matter, 11 (16). pp. 3279-3286. ISSN 1744-683X
Abstract
Lipid bicontinuous cubic phases have attracted enormous interest as bio-compatible scaffolds for use in a wide range of applications including membrane protein crystallisation, drug delivery and biosensing. One of the major bottlenecks that has hindered exploitation of these structures is an inability to create targeted highly swollen bicontinuous cubic structures with large and tunable pore sizes. In contrast, cubic structures found in vivo have periodicities approaching the micron scale. We have been able to engineer and control highly swollen bicontinuous cubic phases of spacegroup Im3m containing only lipids by (a) increasing the bilayer stiffness by adding cholesterol and (b) inducing electrostatic repulsion across the water channels by addition of anionic lipids to monoolein. By controlling the composition of the ternary mixtures we have been able to achieve lattice parameters up to 470 Å, which is 5 times that observed in pure monoolein and nearly twice the size of any lipidic cubic phase reported previously. These lattice parameters significantly exceed the predicted maximum swelling for bicontinuous cubic lipid structures, which suggest that thermal fluctuations should destroy such phases for lattice parameters larger than 300 Å.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) > FSN Colloids and Food Processing (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 04 Apr 2017 15:46 |
Last Modified: | 26 Oct 2020 16:44 |
Published Version: | https://doi.org/10.1039/C5SM00311C |
Status: | Published |
Publisher: | Royal Society of Chemistry |
Identification Number: | 10.1039/C5SM00311C |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:112479 |