Rendeková, J, Ward, TA orcid.org/0000-0003-0937-4554, Šimoničová, L et al. (5 more authors) (2016) Mgm101: A double-duty Rad52-like protein. Cell Cycle, 15 (23). pp. 3169-3176. ISSN 1538-4101
Abstract
Mgm101 has well-characterized activity for the repair and replication of the mitochondrial genome. Recent work has demonstrated a further role for Mgm101 in nuclear DNA metabolism, contributing to an S-phase specific DNA interstrand cross-link repair pathway that acts redundantly with a pathway controlled by Pso2 exonuclease. Due to involvement of FANCM, FANCJ and FANCP homologues (Mph1, Chl1 and Slx4), this pathway has been described as a Fanconi anemia-like pathway. In this pathway, Mgm101 physically interacts with the DNA helicase Mph1 and the MutSα (Msh2/Msh6) heterodimer, but its precise role is yet to be elucidated. Data presented here suggests that Mgm101 functionally overlaps with Rad52, supporting previous suggestions that, based on protein structure and biochemical properties, Mgm101 and Rad52 belong to a family of proteins with similar function. In addition, our data shows that this overlap extends to the function of both proteins at telomeres, where Mgm101 is required for telomere elongation during chromosome replication in rad52 defective cells. We hypothesize that Mgm101 could, in Rad52-like manner, preferentially bind single-stranded DNAs (such as at stalled replication forks, broken chromosomes and natural chromosome ends), stabilize them and mediate single-strand annealing-like homologous recombination event to prevent them from converting into toxic structures.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | (c) 2016, Jana Rendekova, Thomas A. Ward, Lucia Simonicova, Peter H. Thomas, Jozef Nosek, Lubomır Tomaska, Peter J. McHugh, and Miroslav Chovanec. Published with license by Taylor & Francis. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/ 3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted. |
Keywords: | DNA interstrand cross-link repair, Fanconi anemia, Mgm101, mitochondrial DNA, Rad52, telomere, yeast |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 19 Dec 2016 17:26 |
Last Modified: | 05 Oct 2017 16:25 |
Published Version: | https://doi.org/10.1080/15384101.2016.1231288 |
Status: | Published |
Publisher: | Taylor & Francis |
Identification Number: | 10.1080/15384101.2016.1231288 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:109619 |