Torabi, F, Binduraihem, A and Miller, D orcid.org/0000-0002-1709-108X (2017) Sedimentation properties in density gradients correspond with levels of sperm DNA fragmentation, chromatin compaction and binding affinity to hyaluronic acid. Reproductive BioMedicine Online, 34 (3). pp. 298-311. ISSN 1472-6483
Abstract
Mature spermatozoa bind hyaluronic acid in the extracellular matrix via hyaladherins. Immature spermatozoa may be unable to interact because they do not express the appropriate hyaladherins on their surface. Fresh human semen samples were fractionated using differential density gradient centrifugation (DDGC) and the ability of these fractions to bind hyaluronic acid was evaluated. The presence of sperm hyaladherins was also assessed. CD44 was located mainly on the acrosome and equatorial segment and became more restricted to the equatorial segment in capacitated spermatozoa. Hyaluronic acid-TRITC (hyaluronan acid conjugated with tetramethylrhodamine isothiocyanante), a generic hyaluronic-acid-binding reagent, labelled the membrane and the neck region, particularly after capacitation. Sperm populations obtained after DDGC or after interaction with hyaluronic acid were assessed for DNA fragmentation and chromatin maturity. Strong relationships between both measures and sperm sedimentation and hyaluronic-acid-binding profiles were revealed. Capacitation enhanced hyaluronic-acid-binding of both DDGC-pelleted sperm and sperm washed free of seminal fluid. In conclusion, hyaladherins were detected on human sperm and a higher capacity for sperm hyaluronic-acid-binding was shown to correspond with their DDGC sedimentation profiles and with lower levels of DNA fragmentation and better chromatin maturity. Capacitation induced changes in the distribution and presence of hyaladherins may enhance hyaluronic-acid-binding.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 Published by Elsevier Ltd on behalf of Reproductive Healthcare Ltd. This is an author produced version of a paper published in Reproductive BioMedicine Online. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | DNA integrity; chromatin maturity; hyaluronic acid; hyaladherins; capacitation; acrosome reaction |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Leeds Institute of Genetics, Health and Therapeutics (LIGHT) > Division of Reproduction & Early Development Research (Leeds) |
Funding Information: | Funder Grant number Biocoat Inc No Ext Ref Given National Inst for Health Research (NIHR) 11/14/34 |
Depositing User: | Symplectic Publications |
Date Deposited: | 15 Dec 2016 12:25 |
Last Modified: | 15 Dec 2017 01:38 |
Published Version: | https://doi.org/10.1016/j.rbmo.2016.11.011 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.rbmo.2016.11.011 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:109553 |