Jones, G orcid.org/0000-0002-7022-921X, Herbert, A orcid.org/0000-0002-6527-2205, Berry, H orcid.org/0000-0003-2426-1881 et al. (3 more authors) (2017) Decellularization and Characterization of Porcine Superflexor Tendon: A Potential Anterior Cruciate Ligament Replacement. Tissue Engineering Part A, 23 (3-4). pp. 124-134. ISSN 1937-3341
Abstract
The porcine superflexor tendon (SFT) was identified as having appropriate structure and properties for development of a decellularised device for use in ACL reconstruction. SFTs were decellularised using a combination of freeze thaw and washes in hypotonic buffer and 0.1% (w/v) SDS in hypotonic buffer plus proteinase inhibitors followed by nuclease treatment and sterilisation using peracetic acid. The decellularised biological scaffold was devoid of cells and cell remnants and contained only 13 ng.mg-1 (dry weight) residual total DNA. Immunohistochemistry showed retention of collagens type I and III and tenascin-C. Quantitative analysis of sulphated sugar and hydroxyproline content revealed a loss of glycosaminoglycans compared to native tissue but no loss of collagen. The decellularised SFT was biocompatible in vitro and in vivo following implantation in a mouse subcutaneous model for 12 weeks.
Uniaxial tensile testing to failure indicated that the gross material properties of decellularised SFT were not significantly different to native tissue. Decellularised SFTs had an ultimate tensile strength of 61.8 ± 10.3 MPa (± 95 % confidence limits), a failure strain of 0.29 ± 0.04 and a Young’s modulus of the collagen phase of 294.1 ± 61.9 MPa.
Analysis of the presence of the alpha-Gal (galactose-α-1,3-galactose) epitope by immunohistochemistry, lectin binding and antibody absorption assay indicated that the epitope was reduced but still present post decellularisation. This is discussed in light of the potential role of non-cellular alpha-Gal in the acceleration of wound healing and tissue regeneration in the presence of antibodies to alpha-Gal.
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Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © Gemma Jones, et al., 2016; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
Keywords: | decellularization; tendon; anterior cruciate ligament |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Mechanical Engineering (Leeds) > Institute of Medical and Biological Engineering (iMBE) (Leeds) The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biomedical Sciences (Leeds) |
Funding Information: | Funder Grant number Wellcome Trust 088908/Z/09/Z Leeds Teaching Hospitals NHS Trust Not Known NIHR National Inst Health Research NONE GIVEN NIHR National Inst Health Research PO - 427791 EU - European Union 267114 |
Depositing User: | Symplectic Publications |
Date Deposited: | 26 Oct 2016 10:08 |
Last Modified: | 12 Dec 2024 14:41 |
Published Version: | https://doi.org/10.1089/ten.tea.2016.0114 |
Status: | Published |
Publisher: | Mary Ann Liebert |
Identification Number: | 10.1089/ten.tea.2016.0114 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:106269 |
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