Hoffmann, Sara, Danesh-Azari, Hamid-Reza, Spandolf, Claudia et al. (3 more authors) (2016) Structure-Guided Redesign of CYP153AM.aq for the Improved Terminal Hydroxylation of Fatty Acids. ChemCatChem. 3234–3239. ISSN 1867-3899
Abstract
The structure of a P450 ω-hydroxylase bound to its fatty acid product was determined, which revealed a narrow substrate tunnel that leads to the heme. The introduction of an arginine side chain in proximity to the carboxyl group of the fatty acid led to a reduced KM value for dodecanoic acid, which suggests the importance of an anchoring point in the active site. An increase in the flexibility of the substrate recognition region was also engineered, which resulted in a threefold improved product formation.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016, Wiley-VCH Verlag GmbH & Co. K. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details. |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) |
Depositing User: | Pure (York) |
Date Deposited: | 08 Sep 2016 14:05 |
Last Modified: | 08 Feb 2025 00:20 |
Published Version: | https://doi.org/10.1002/cctc.201600680 |
Status: | Published online |
Refereed: | Yes |
Identification Number: | 10.1002/cctc.201600680 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:104414 |