A high-throughput colourimetric method for the determination of pH in crystallization screens

Abstract

The crystallization of proteins is dependent on the careful control of numerous parameters, one of these being pH. The pH of crystallization is generally reported as that of the buffer; however, the true pH has been found to be as many as four pH units away. Measurement of pH with a meter is time-consuming and requires the reformatting of the crystallization solution. To overcome this, a high-throughput method for pH determination of buffered solutions has been developed with results comparable to those of a pH meter.

Metadata

Authors/Creators:	Kirkwood, Jobie Wilson, Julie https://orcid.org/0000-0002-5171-8480 O'keefe, Simon https://orcid.org/0000-0001-5957-2474 Hargreaves, David
Copyright, Publisher and Additional Information:	© 2014 International Union of Crystallography. Reproduced in accordance with the publisher's self-archiving policy.
Keywords:	absorbance, bromothymol blue, colourimetry, crystallization, indicator dye, pH, spectrophotometry
Dates:	Published: 1 January 2014
Institution:	The University of York
Academic Units:	The University of York > Faculty of Sciences (York) > Computer Science (York) The University of York The University of York > Faculty of Sciences (York) > Chemistry (York) The University of York > Faculty of Sciences (York) > Mathematics (York)
Depositing User:	Pure (York)
Date Deposited:	10 Dec 2015 13:57
Last Modified:	01 Feb 2024 00:15
Published Version:	https://doi.org/10.1107/S1399004714014011
Status:	Published
Refereed:	Yes
Identification Number:	https://doi.org/10.1107/S1399004714014011
Related URLs:	http://www.scopus.com/inward/record.url?...

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A high-throughput colourimetric method for the determination of pH in crystallization screens

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