Thangsunan, P, Lal, N, Tiede, C et al. (8 more authors) (2021) Affimer-based impedimetric biosensors for fibroblast growth factor receptor 3 (FGFR3): a novel tool for detection and surveillance of recurrent bladder cancer. Sensors and Actuators B: Chemical, 326. 128829. ISSN 0925-4005
Abstract
Fibroblast growth factor receptor 3 (FGFR3) is a transmembrane tyrosine kinase protein in the fibroblast growth factor receptor family, which plays a key role in many biological processes. Over-expression and activating mutations in FGFR3 are frequent in non-invasive bladder cancer, highlighting this protein as a potential biomarker for recurrent bladder cancer detection. Affimer reagents isolated against recombinant FGFR3 were assessed for their affinity using double-sandwich ELISA and SPR. Anti-FGFR3-14 and FGFR3-21 Affimer proteins showed strong binding to FGFR3 and were used for fabrication of impedimetric electrochemical biosensors. A decrease in impedance was observed when the sensors were exposed to increasing concentrations of FGFR3. The successful impedimetric biosensors were capable of detecting sub-pM to nM concentrations of recombinant FGFR3 protein in phosphate buffered saline as well as in synthetic urine.
Metadata
Downloads
Filename: FGFR3_Publication_7thDraft.pdf
Licence: CC-BY-NC-ND 4.0
Filename: SupplementaryData_FGFR3Paper_2 (1).pdf
Licence: CC-BY-NC-ND 4.0