Tronci, G orcid.org/0000-0002-9426-4220, Yin, J, Holmes, RA et al. (3 more authors) (2016) Protease-sensitive atelocollagen hydrogels promote healing in a diabetic wound model. Journal of Materials Chemistry B, 4 (45). pp. 7249-7258. ISSN 2050-750X
Abstract
The design of exudate-managing wound dressings is an established route to accelerated healing, although such design remains a challenge from material and manufacturing standpoints. Aiming towards the clinical translation of knowledge gained in vitro with highly swollen rat tail collagen hydrogels, this study investigated the healing capability in a diabetic mouse wound model of telopeptide-free, protease-inhibiting collagen networks. 4-vinylbenzylation and UV irradiation of type I atelocollagen (AC) led to hydrogel networks with chemical and macroscopic properties comparable to previous collagen analogues, attributable to similar lysine content and dichroic properties. After 4 days in vitro, hydrogels induced nearly 50 RFU% reduction in matrix metalloproteinase (MMP)-9 activity, whilst showing less than 20 wt.-% weight loss. After 20 days in vivo, dry networks promoted 99% closure of 10×10 mm full thickness wounds and accelerated neo-dermal tissue formation compared to Mepilex®. This collagen system can be equipped with multiple, customisable properties and functions key to personalised chronic wound care.
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Copyright, Publisher and Additional Information: | © 2016, Royal Society of Chemistry. This is an author produced version of a paper published in Journal of Materials Chemistry B. Uploaded in accordance with the publisher's self-archiving policy. | ||||||||||||
Keywords: | Atelocollagen; hydrogel wound dressings; chronic wounds; MMPs | ||||||||||||
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Institution: | The University of Leeds | ||||||||||||
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Dentistry (Leeds) > Oral Biology (Leeds) The University of Leeds > Faculty of Arts, Humanities and Cultures (Leeds) > School of Design (Leeds) |
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Depositing User: | Symplectic Publications | ||||||||||||
Date Deposited: | 19 Oct 2016 09:24 | ||||||||||||
Last Modified: | 19 Oct 2017 17:30 | ||||||||||||
Published Version: | https://doi.org/10.1039/C6TB02268E | ||||||||||||
Status: | Published | ||||||||||||
Publisher: | Royal Society of Chemistry | ||||||||||||
Identification Number: | https://doi.org/10.1039/C6TB02268E |