Stockley, Peter G., White, Simon J., Dykeman, Eric Charles et al. (11 more authors) (2016) Bacteriophage MS2 Genomic RNA Encodes an Assembly Instruction Manual for Its Capsid. Bacteriophage. e1157666. ISSN 2159-7081
Abstract
Using RNA-coat protein crosslinking we have shown that the principal RNA recognition surface on the interior of infectious MS2 virions overlaps with the known peptides that bind the high affinity translational operator, TR, within the phage genome. The data also reveal the sequences of genomic fragments in contact with the coat protein shell. These show remarkable overlap with previous predictions based on the hypothesis that virion assembly is mediated by multiple sequences-specific contacts at RNA sites termed Packaging Signals (PSs). These PSs are variations on the TR stem-loop sequence and secondary structure. They act co-operatively to regulate the dominant assembly pathway and ensure cognate RNA encapsidation. In MS2, they also trigger conformational change in the dimeric capsomere creating the A/B quasi-conformer, 60 of which are needed to complete the T=3 capsid. This is the most compelling demonstration to date that this ssRNA virus, and by implications potentially very many of them, assemble via a PS-mediated assembly mechanism.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 The Author(s). |
Keywords: | RNA bacteriophage,,RNA packaging signals,virion assembly |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) The University of York > Faculty of Sciences (York) > Mathematics (York) |
Funding Information: | Funder Grant number EPSRC EP/K028286/1 BBSRC (BIOTECHNOLOGY AND BIOLOGICAL SCIENCES RESEARCH COUNCIL) BB/L021803/1 |
Depositing User: | Pure (York) |
Date Deposited: | 05 May 2016 16:27 |
Last Modified: | 23 Jan 2025 00:07 |
Published Version: | https://doi.org/10.1080/21597081.2016.1157666 |
Status: | Published online |
Refereed: | Yes |
Identification Number: | 10.1080/21597081.2016.1157666 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:99359 |