Nguyen, V., Karunakaran, E., Collins, G. et al. (1 more author) (2016) Physicochemical analysis of initial adhesion and biofilm formation of Methanosarcina barkeri on polymer support material. Colloids and Surfaces B: Biointerfaces, 143. pp. 518-525. ISSN 0927-7765
Abstract
The retention of selective biofilms of Methanosarcina species within anaerobic digesters could reduce start-up times and enhance the efficiency of the process in treating high-strength domestic sewage. The objective of the study was to examine the effect of the surface characteristics of six common polymer support materials on the initial adhesion of the model methanogen, Methanosarcina barkeri, and to assess the potential of these support materials as selective biofilm carriers. Results from both the initial adhesion tests and extended DLVO (xDLVO) model correlated with each other, with PVC (12% surface coverage/mm(2)), PTFE (6% surface coverage/mm(2)), and PP (6% surface coverage/mm(2)), shown to be the better performing support materials for initial adhesion, as well as subsequent biofilm formation by M. barkeri after 72h. Experimental results of these three support materials showed that the type of material strongly influenced the extent of adhesion from M. barkeri (p<0.0001), and the xDLVO model was able to explain the results in these environmental conditions. Therefore, DLVO physicochemical forces were found to be influential on the initial adhesion of M. barkeri. Scanning electron microscopy suggested that production of extracellular polymeric substances (EPS) from M. barkeri could facilitate further biofilm development. This study highlights the potential of using the xDLVO model to rapidly identify suitable materials for the selective adhesion of M. barkeri, which could be beneficial in both the start-up and long-term phases of anaerobic digestion.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 Elsevier. This is an author produced version of a paper subsequently published in Colloids and Surfaces B: Biointerfaces. Uploaded in accordance with the publisher's self-archiving policy. Article available under the terms of the CC-BY-NC-ND licence (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
Keywords: | Biofilm formation; DLVO model; Initial adhesion; Methanosarcina barkeri; Physicochemical; Support material |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Chemical and Biological Engineering (Sheffield) |
Funding Information: | Funder Grant number ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL (EPSRC) EP/D031311/1 BIOTECHNOLOGY AND BIOLOGICAL SCIENCES RESEARCH COUNCIL (BBSRC) BB/C505391/1 ROYAL SOCIETY UNSPECIFIED NUFFIELD FOUNDATION NAL/00694/G ROYAL SOCIETY 574006.G503/24320/SM LEVERHULME TRUST (THE) F00118AG EUROPEAN COMMISSION - FP6/FP7 MTKD-CT-2006-042444 YORKSHIRE WATER SERVICES NONE ROYAL SOCIETY None ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL (EPSRC) EP/H023488/1 ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL (EPSRC) EP/J004871/1 ROYAL ACADEMY OF ENGINEERING (THE) ILB/LB/ITG 03-715 THAMES WATER UTILITIES PLC SHEFF- DE03OV.05- 003 ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL (EPSRC) EP/I016589/1 ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL (EPSRC) GR/S72467/01 PROCTOR & GAMBLE TECHNICAL CENTRES LTD UNSPECIFIED ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL (EPSRC) EP/D031311/1 ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL (EPSRC) EP/N010124/1 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 13 May 2016 11:49 |
Last Modified: | 16 Nov 2017 14:23 |
Published Version: | http://dx.doi.org/10.1016/j.colsurfb.2016.03.042 |
Status: | Published |
Publisher: | Elsevier |
Refereed: | Yes |
Identification Number: | 10.1016/j.colsurfb.2016.03.042 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:99099 |