Jensen, H.S., Sekar, R., Shepherd, W.J. orcid.org/0000-0003-4434-9442 et al. (3 more authors) (2016) Spatial and temporal variability of bacterial communities within a combined sewer system. MicrobiologyOpen, 5 (4). pp. 616-625. ISSN 2045-8827
Abstract
This study describes the temporal and spatial variability of bacterial communities within a combined sewer system in England. Sampling was conducted over 9 months in a sewer system with intensive monitoring of hydraulic conditions. The bacterial communities were characterized by 16S rRNA gene-targeted terminal restriction fragment length polymorphism analysis. These data were related to the hydraulic data as well as the sample type, location, and time. Temporal and spatial variation was observed between and within wastewater communities and biofilm communities. The bacterial communities in biofilm were distinctly different from the communities in wastewater and exhibited greater spatial variation, while the wastewater communities exhibited variability between different months of sampling. This study highlights the variation of bacterial communities between biofilm and wastewater, and has shown both spatial and temporal variations in bacterial communities in combined sewers. The temporal variation is of interest for in-sewer processes, for example, sewer odor generation, as field measurements for these processes are often carried out over short durations and may therefore not capture the influence of this temporal variation of the bacterial communities.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/4.0/ |
Keywords: | Bacteria; T-RFLP analysis; environmental biofilms; in-sewer processes; sewer systems; wastewater |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Chemical and Biological Engineering (Sheffield) The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Civil and Structural Engineering (Sheffield) |
Funding Information: | Funder Grant number ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL (EPSRC) EP/D031311/1 BIOTECHNOLOGY AND BIOLOGICAL SCIENCES RESEARCH COUNCIL (BBSRC) BB/C505391/1 ROYAL SOCIETY UNSPECIFIED NUFFIELD FOUNDATION NAL/00694/G ROYAL SOCIETY 574006.G503/24320/SM LEVERHULME TRUST (THE) F00118AG ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL (EPSRC) EP/E053556/1 YORKSHIRE WATER SERVICES NONE ROYAL SOCIETY None ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL (EPSRC) EP/H023488/1 ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL (EPSRC) EP/J004871/1 ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL (EPSRC) EP/J00538X/1 ROYAL ACADEMY OF ENGINEERING (THE) ILB/LB/ITG 03-715 ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL (EPSRC) EP/I029346/1 ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL (EPSRC) EP/I016589/1 ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL (EPSRC) GR/S72467/01 PROCTOR & GAMBLE TECHNICAL CENTRES LTD UNSPECIFIED ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL (EPSRC) EP/D031311/1 ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL (EPSRC) EP/I031812/1 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 28 Apr 2016 08:18 |
Last Modified: | 18 Nov 2016 17:10 |
Published Version: | http://dx.doi.org/10.1002/mbo3.356 |
Status: | Published |
Publisher: | Wiley Open Access |
Refereed: | Yes |
Identification Number: | 10.1002/mbo3.356 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:99098 |