Petrache, AI, Machin, DC, Williamson, DJ et al. (2 more authors) (2016) Sortase-mediated labelling of lipid nanodiscs for cellular tracing. Molecular BioSystems, 12 (6). pp. 1760-1763. ISSN 1742-206X
Abstract
Lipid nanodiscs have broad applications in membrane protein assays, biotechnology and materials science. Chemical modification of the nanodiscs to expand their functional attributes is generally desirable for all of these uses. We present a method for site-selective labelling of the N-terminus of the nanodisc’s membrane scaffold protein (MSP) using the Sortase A protein. Labelling of the MSP was achieved when assembled within the lipid nanodisc architecture, demonstrating that this method can be used as a retrofit approach to modification of preformed nanodiscs before or during application. We label the MSP with a fluorescent fluorescein moiety and use them to image nanodisc uptake into HeLa cells. The Sortase A labelling method could be employed as a general approach to labelling nanodiscs with application-specific functionalities.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/). |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) > Organic Chemistry (Leeds) The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) > Physical Chemistry (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 20 Apr 2016 10:40 |
Last Modified: | 11 Apr 2018 13:03 |
Published Version: | http://dx.doi.org/10.1039/C6MB00126B |
Status: | Published |
Publisher: | Royal Society of Chemistry |
Identification Number: | 10.1039/C6MB00126B |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:98609 |