Saidi, S., Bouri, F., Lencel, P. et al. (9 more authors) (2011) IL-33 is expressed in human osteoblasts, but has no direct effect on bone remodeling. Cytokine, 53 (3). pp. 347-354. ISSN 1043-4666
Abstract
The aim of the present study was to investigate the potential role of the recently discovered IL-1 family member IL-33 in bone remodeling. Our results indicate that IL-33 mRNA is expressed in osteocytes in non-inflammatory human bone. Moreover, IL-33 levels are increased by TNF-α and IL-1β in human bone marrow stromal cells, osteoblasts and adipocytes obtained from three healthy donors. Experiments with the inhibitor GW-9662 suggested that expression of IL-33, in contrast to that of IL-1β, is not repressed by PPARγ likely explaining why IL-33, but not IL-1β, is expressed in adipocytes. The IL-33 receptor ST2L is not constitutively expressed in human bone marrow stromal cells, osteoblasts or CD14-positive monocytes, and IL-33 has no effect on these cells. In addition, although ST2L mRNA is induced by TNF-α and IL-1β in bone marrow stromal cells, IL-33 has the same effects as TNF-α and IL-1β, and, therefore, the biological activity of IL-33 may be redundant in this system. In agreement with this hypothesis, MC3T3-E1 osteoblast-like cells constitutively express ST2L mRNA, and IL-33 and TNF-α/IL-1β similarly decrease osteocalcin RNA levels in these cells. In conclusion, our results suggest that IL-33 has no direct effects on normal bone remodeling.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2010 Elsevier Ltd. All rights reserved. |
Keywords: | IL-33; Bone remodeling; Inflammation; IL-1; INTERLEUKIN-1 RECEPTOR ANTAGONIST; PROLIFERATOR-ACTIVATED RECEPTOR; ACCESSORY PROTEIN; MAST-CELLS; TNF-ALPHA; IN-VIVO; INFLAMMATORY MEDIATORS; OVARIECTOMIZED MICE; OSTEOSARCOMA CELLS; INDUCED ARTHRITIS |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > The Medical School (Sheffield) > Division of Genomic Medicine (Sheffield) > Department of Oncology and Metabolism (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 06 May 2016 08:18 |
Last Modified: | 10 Jul 2016 14:19 |
Published Version: | http://dx.doi.org/10.1016/j.cyto.2010.11.021 |
Status: | Published |
Publisher: | Elsevier |
Refereed: | Yes |
Identification Number: | 10.1016/j.cyto.2010.11.021 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:98194 |