Wu, Z., Jiang, G., Xiang, P. et al. (2 more authors) (2008) Purification and characterization of trypsin-like enzymes from North Pacific krill (Euphausia pacifica). Biotechnology Letters, 30 (1). pp. 67-72. ISSN 0141-5492
Abstract
Two trypsin-like enzymes (TLEs) were purified from North Pacific krill (Euphausia pacifica) by ammonium sulfate precipitation, ion-exchange and gel-filtration chromatography. The purified enzymes were identified as trypsins by LC-ESI-MS/MS analysis. The relative molecular mass of TLE I and TLE II were 33 and 32.3 kDa, respectively, with isoelectric points of 4.5 and 4.3, respectively. The TLEs showed excellent thermal stable in the crude extract and the purified TLEs were active over a wide pH (6.0-11.0) and temperature (10-70 degrees C) range. Compared with trypsins from other organisms, the purified TLEs had physiological efficiencies of 1.6-6.7-fold. The difference in Arg, Ile and Asp content might explain why E. pacifica TLEs have good thermal stability and physiological efficiency.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © Springer Science+Business Media B.V. 2007. |
Keywords: | Amino Acid Sequence; Animals; Enzyme Activation; Enzyme Stability; Euphausiacea; Molecular Sequence Data; Trypsin |
Dates: |
|
Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > Department of Human Metabolism (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 06 May 2016 08:59 |
Last Modified: | 06 Jul 2016 15:48 |
Published Version: | http://dx.doi.org/10.1007/s10529-007-9511-6 |
Status: | Published |
Publisher: | Springer Verlag |
Refereed: | Yes |
Identification Number: | 10.1007/s10529-007-9511-6 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:97684 |