Orriss, I.R., Wang, N., Burnstock, G. et al. (4 more authors) (2011) The P2Y(6) Receptor Stimulates Bone Resorption by Osteoclasts. Endocrinology, 152 (10). pp. 3706-3716. ISSN 0013-7227
Abstract
Accumulating evidence indicates that extracellular nucleotides, signaling through P2 receptors, play a significant role in bone remodeling. Osteoclasts (the bone-resorbing cell) and osteoblasts (the bone-forming cell) display expression of the G protein-coupled P2Y6 receptor, but the role of this receptor in modulating cell function is unclear. Here, we demonstrate that extracellular UDP, acting via P2Y6 receptors, stimulates the formation of osteoclasts from precursor cells, while also enhancing the resorptive activity of mature osteoclasts. Furthermore, osteoclasts derived from P2Y6 receptor-deficient (P2Y6R−/−) animals displayed defective function in vitro. Using dual energy x-ray absorptiometry scanning and microcomputed tomographic analysis we showed that P2Y6R−/− mice have increased bone mineral content, cortical bone volume, and cortical thickness in the long bones and spine, whereas trabecular bone parameters were unaffected. Histomorphometric analysis showed the perimeter of the bone occupied by osteoclasts on the endocortical and trabecular surfaces was decreased in P2Y6R−/− mice. Taken together these results show the P2Y6 receptor may play an important role in the regulation of bone cell function in vivo. - See more at: http://press.endocrine.org/doi/abs/10.1210/en.2011-1073#sthash.NxF2CovR.dpuf
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | Copyright © 2011 by The Endocrine Society |
Keywords: | SMOOTH-MUSCLE-CELLS; NF-KAPPA-B; NUCLEOTIDE RECEPTOR; IN-VITRO; P2X7 RECEPTORS; P2Y6 RECEPTOR; ATP; EXPRESSION; ACTIVATION; RELEASE |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > Department of Human Metabolism (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 09 May 2016 09:46 |
Last Modified: | 11 Jun 2016 18:05 |
Published Version: | http://dx.doi.org/10.1210/en.2011-1073 |
Status: | Published |
Publisher: | Endocrine Society |
Refereed: | Yes |
Identification Number: | 10.1210/en.2011-1073 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:97683 |