Bilancia, C.G., Winkelman, J.D., Tsygankov, D. et al. (9 more authors) (2014) Enabled Negatively Regulates Diaphanous-Driven Actin Dynamics In Vitro and In Vivo. Developmental Cell , 28 (4). pp. 394-408. ISSN 1534-5807
Abstract
Actin regulators facilitate cell migration by controlling cell protrusion architecture and dynamics. As the behavior of individual actin regulators becomes clear, we must address why cells require multiple regulators with similar functions and how they cooperate to create diverse protrusions. We characterized Diaphanous (Dia) and Enabled (Ena) as a model, using complementary approaches: cell culture, biophysical analysis, and Drosophila morphogenesis. We found that Dia and Ena have distinct biochemical properties that contribute to the different protrusion morphologies each induces. Dia is a more processive, faster elongator, paralleling the long, stable filopodia it induces in vivo, while Ena promotes filopodia with more dynamic changes in number, length, and lifetime. Acting together, Ena and Dia induce protrusions distinct from those induced by either alone, with Ena reducing Dia-driven protrusion length and number. Consistent with this, EnaEVH1 binds Dia directly and inhibits DiaFH1FH2-mediated nucleation in vitro. Finally, Ena rescues hemocyte migration defects caused by activated Dia.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2014 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
Keywords: | Actins; Animals; Carrier Proteins; Cell Movement; DNA-Binding Proteins; Drosophila Proteins; Drosophila melanogaster; Hemocytes; Morphogenesis; Pseudopodia |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > Department of Infection and Immunity (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 13 Apr 2016 14:31 |
Last Modified: | 13 Apr 2016 14:31 |
Published Version: | http://dx.doi.org/10.1016/j.devcel.2014.01.015 |
Status: | Published |
Publisher: | Elsevier (Cell Press) |
Refereed: | Yes |
Identification Number: | 10.1016/j.devcel.2014.01.015 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:97600 |
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