Foster, RJ, Damion, RA, Baboolal, TG orcid.org/0000-0003-4444-0318 et al. (2 more authors) (2016) A nuclear magnetic resonance study of water in aggrecan solutions. Royal Society Open Science, 3 (3). 150705.
Abstract
Aggrecan, a highly-charged macromolecule found in articular cartilage, was investigated in aqueous salt solutions with proton Nuclear Magnetic Resonance. The longitudinal and transverse relaxation rates were determined at two different field strengths, 9.4 T and 0.5 T, for a range of temperatures and aggrecan concentrations. The diffusion coefficients of the water molecules were also measured as a function of temperature and aggrecan concentration, using a pulsed field gradient technique at 9.4 T. Assuming an Arrhenius relationship, the activation energies for the various relaxation processes and the translational motion of the water molecules were determined from temperature dependencies as a function of aggrecan concentration in the range 0 – 5.3 % w/w. The longitudinal relaxation rate and inverse diffusion coefficient were approximately equally dependent on concentration and only increased by ≤ 20% from that of the salt solution. The transverse relaxation rate at high field demonstrated greatest concentration dependence, changing by an order of magnitude across the concentration range examined. We attribute this primarily to chemical exchange. Activation energies appeared to be approximately independent of aggrecan concentration, except for that of the low-field transverse relaxation rate, which decreased with concentration.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. |
Keywords: | Aggrecan; Glycosaminoglycan; Relaxation; Diffusion; Activation energy; Chemical exchange |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemical & Process Engineering (Leeds) The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Physics and Astronomy (Leeds) > Soft Matter Physics (Leeds) |
Funding Information: | Funder Grant number Wellcome Trust 088908/Z/09/Z Wellcome Trust 088908/Z/09/Z |
Depositing User: | Symplectic Publications |
Date Deposited: | 08 Jul 2016 13:57 |
Last Modified: | 11 Apr 2018 13:21 |
Published Version: | https://dx.doi.org/10.1098/rsos.150705 |
Status: | Published |
Publisher: | The Royal Society |
Identification Number: | 10.1098/rsos.150705 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:97220 |
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