Smith, L.E., Hearnden, V. orcid.org/0000-0003-0838-7783, Lu, Z. et al. (6 more authors) (2011) Evaluating the use of optical coherence tomography for the detection of epithelial cancers in vitro. Journal of Biomedical Optics, 16 (11). 116015. ISSN 1083-3668
Abstract
Optical coherence tomography (OCT) is a noninvasive imaging methodology that is able to image tissue to depths of over 1 mm. Many epithelial conditions, such as melanoma and oral cancers, require an invasive biopsy for diagnosis. A noninvasive, real-time, point of care method of imaging depth-resolved epithelial structure could greatly improve early diagnosis and long-term monitoring in patients. Here, we have used tissue-engineered (TE) models of normal skin and oral mucosa to generate models of melanoma and oral cancer. We have used these to determine the ability of OCT to image epithelial differences in vitro. We report that while in vivo OCT gives reasonable depth information for both skin and oral mucosa, in vitro the information provided is less detailed but still useful. OCT can provide reassurance on the development of TE models of skin and oral mucosa as they develop in vitro. OCT was able to detect the gross alteration in the epithelium of skin and mucosal models generated with malignant cell lines but was less able to detect alteration in the epithelium of TE models that mimicked oral dysplasia or, in models where tumor cells had penetrated into the dermis.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). Reproduced in accordance with the publisher's self-archiving policy. |
Keywords: | optical coherence tomography; melanoma; oral cancer; noninvasive imaging; tissue engineering; skin; oral dysplasia |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Computer Science (Sheffield) The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Electronic and Electrical Engineering (Sheffield) The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Materials Science and Engineering (Sheffield) The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > School of Clinical Dentistry (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 19 Apr 2016 14:32 |
Last Modified: | 19 Apr 2016 14:32 |
Published Version: | http://dx.doi.org/10.1117/1.3652708 |
Status: | Published |
Publisher: | Society of Photo-optical Instrumentation Engineers |
Refereed: | Yes |
Identification Number: | 10.1117/1.3652708 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:97059 |