Green, N. orcid.org/0000-0001-5413-0642, Huang, Q.Z., Khan, L. et al. (4 more authors) (2010) The Development and Characterization of an Organotypic Tissue-Engineered Human Esophageal Mucosal Model. Tissue Engineering: Part A, 16 (3). pp. 1053-1064. ISSN 1937-3341
Abstract
There is a demand for a reliable three-dimensional tissue-engineered model of the esophageal mucosa for use as an experimental platform for investigating esophageal epithelial biology and the pathogenesis of esophageal neoplasia and precursor lesions such as Barrett's metaplasia. A number of models have been described, but there has been little systematic assessment of the different approaches, making selection of a preferred platform difficult. This study assesses the properties of organotypic cultures using four different scaffolds (human esophageal matrix, porcine esophageal matrix, human dermal matrix, and collagen) and two different epithelial cell types (primary human esophageal squamous cells and the Het-1A esophageal squamous cell line). Human esophageal matrix and dermis did not give consistent results, but porcine esophageal matrix and collagen proved more reliable and were studied in greater detail. Both matrices supported the formation of a mature stratified epithelium that was similar to that of the normal human esophagus, demonstrated by Ki67, CK4, CK14, and involucrin staining. However, collagen showed reduced epithelial adherence, while fibroblast penetration into the porcine matrix was poor. Composite cultures using Het-1A cells formed a hyperproliferative epithelium with no evidence of differentiation. We propose human esophageal squamous cells seeded onto porcine esophageal matrix as the preferred model of the normal human esophagus.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2010 Mary Ann Liebert, Inc. publishers. This is an author produced version of a paper subsequently published in Tissue Engineering Part A. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | epithelial-cells; in-vitro; clinical-use; acellular matrix; fibroblasts; keratinocytes; acid; skin; differentiation; regeneration |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Materials Science and Engineering (Sheffield) The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > The Medical School (Sheffield) > Division of Genomic Medicine (Sheffield) > Department of Oncology and Metabolism (Sheffield) The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) The University of Sheffield > Sheffield Teaching Hospitals |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 08 Sep 2016 14:40 |
Last Modified: | 25 Oct 2016 13:02 |
Published Version: | https://dx.doi.org/10.1089/ten.tea.2009.0217 |
Status: | Published |
Publisher: | Mary Ann Liebert |
Refereed: | Yes |
Identification Number: | 10.1089/ten.tea.2009.0217 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:97006 |