Hughes, L, Carton, R, Minguzzi, S et al. (4 more authors) (2015) An active second dihydrofolate reductase enzyme is not a feature of rat and mouse, but they do have activity in their mitochondria. FEBS Letters, 589 (15). pp. 1855-1862. ISSN 0014-5793
Abstract
The identification of a second functional dihydrofolate reductase enzyme in humans, DHFRL1, led us to consider whether this is also a feature of rodents. We demonstrate that dihydrofolate reductase activity is also a feature of the mitochondria in both rat and mouse but this is not due to a second enzyme. While our phylogenetic analysis revealed that RNA-mediated DHFR duplication events did occur across the mammal tree, the duplicates in brown rat and mouse are likely to be processed pseudogenes. Humans have evolved the need for two separate enzymes while laboratory rats and mice have just one.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Keywords: | Dihydrofolate reductase; Rat; Mouse; Mitochondria; Folic acid; R. rattus; R. norvegicus |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 02 Sep 2016 11:36 |
Last Modified: | 04 Nov 2016 07:35 |
Published Version: | https://dx.doi.org/10.1016/j.febslet.2015.05.017 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1016/j.febslet.2015.05.017 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:96711 |