Thompson, RF, Walker, M, Siebert, CA et al. (2 more authors) (2016) An introduction to sample preparation and imaging by cryo-electron microscopy for structural biology. Methods, 100. pp. 3-15. ISSN 1046-2023
Abstract
Transmission electron microscopy (EM) is a versatile technique that can be used to image biological specimens ranging from intact eukaryotic cells to individual proteins greater than 150 kDa. There are several strategies for preparing samples for imaging by EM, including negative staining and cryogenic freezing. In the last few years, cryo-EM has undergone a 'resolution revolution', owing to both advances in imaging hardware, image processing software, and improvements in sample preparation, leading to growing number of researchers using cryo-EM as a research tool. However, cryo-EM is still a rapidly growing field, with unique challenges. Here, we summarise considerations for imaging of a range of specimens from macromolecular complexes to cells using EM.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
Keywords: | Electron microscopy |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biomedical Sciences (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 11 Mar 2016 11:42 |
Last Modified: | 11 Apr 2018 13:44 |
Published Version: | http://dx.doi.org/10.1016/j.ymeth.2016.02.017 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.ymeth.2016.02.017 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:96258 |