Feichtinger, G (2016) Ultrasound-mediated gene transfer (sonoporation) in fibrin-based matrices: potential for use in tissue regeneration. Journal of Tissue Engineering and Regenerative Medicine, 10 (1). pp. 29-39. ISSN 1932-6254
Abstract
It has been suggested that gene transfer into donor cells is an efficient and practical means of locally supplying requisite growth factors for applications in tissue regeneration. Here we describe, for the first time, an ultrasound-mediated system that can non-invasively facilitate gene transfer into cells entrapped within fibrin-based matrices. Since ultrasound-mediated gene transfer is enhanced using microbubbles, we compared the efficacy of neutral and cationic forms of these reagents on the ultrasound-stimulated gene transfer process in gel matrices. In doing so we demonstrated the beneficial effects associated with the use of cationic microbubble preparations that interact directly with cells and nucleic acid within matrices. In some cases, gene expression was increased two-fold in gel matrices when cationic microbubbles were compared with neutral microbubbles. In addition, incorporating collagen into fibrin gels yielded a 25-fold increase in gene expression after application of ultrasound to microbubble-containing matrices. We suggest that this novel system may facilitate non-invasive temporal and spatial control of gene transfer in gel-based matrices for the purposes of tissue regeneration. Copyright © 2013 John Wiley & Sons, Ltd.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2013 John Wiley & Sons, Ltd. This is the peer reviewed version of the following article: Nomikou, N., Feichtinger, G. A., Redl, H., and McHale, A. P. (2016) Ultrasound-mediated gene transfer (sonoporation) in fibrin-based matrices: potential for use in tissue regeneration. J Tissue Eng Regen Med, 10: 29–39; which has been published in final form at https://dx.doi.org/10.1002/term.1730. This article may be used for non-commercial purposes in accordance with the Wiley Terms and Conditions for Self-Archiving. |
Keywords: | sonoporation; tissue engineering; gene transfer; microbubbles; fibrin; collagen |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Dentistry (Leeds) > Oral Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 10 Aug 2016 10:01 |
Last Modified: | 22 Jan 2018 09:49 |
Published Version: | https://dx.doi.org/10.1002/term.1730 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1002/term.1730 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:96177 |