Stockley, PG orcid.org/0000-0002-1360-2751, White, SJ, Dykeman, E et al. (11 more authors) (2016) Bacteriophage MS2 genomic RNA encodes an assembly instruction manual for its capsid. Bacteriophage, 6 (1). ISSN 2159-7073
Abstract
Using RNA-coat protein crosslinking we have shown that the principal RNA recognition surface on the interior of infectious MS2 virions overlaps with the known peptides that bind the high affinity translational operator, TR, within the phage genome. The data also reveal the sequences of genomic fragments in contact with the coat protein shell. These show remarkable overlap with previous predictions based on the hypothesis that virion assembly is mediated by multiple sequences-specific contacts at RNA sites termed Packaging Signals (PSs). These PSs are variations on the TR stem-loop sequence and secondary structure. They act co-operatively to regulate the dominant assembly pathway and ensure cognate RNA encapsidation. In MS2, they also trigger conformational change in the dimeric capsomere creating the A/B quasi-conformer, 60 of which are needed to complete the T=3 capsid. This is the most compelling demonstration to date that this ssRNA virus, and by implications potentially very many of them, assemble via a PS-mediated assembly mechanism.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 Peter G. Stockley, Simon J. White, Eric Dykeman, Iain Manfield, Ottar Rolfsson, Nikesh Patel, Richard Bingham, Amy Barker, Emma Wroblewski, Rebecca Chandler-Bostock, Eva U. Weiß, Neil A. Ranson, Roman Tuma, and Reidun Twarock. Published with license by Taylor & Francis. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Keywords: | RNA bacteriophage; RNA packaging signals; virion assembly |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) |
Funding Information: | Funder Grant number BBSRC BB/L022095/1 Wellcome Trust 089310/Z/09/Z Wellcome Trust 090932/Z/09/Z BBSRC BB/J00667X/1 EPSRC EP/K027689/1 |
Depositing User: | Symplectic Publications |
Date Deposited: | 07 Mar 2016 12:58 |
Last Modified: | 10 Apr 2017 16:11 |
Published Version: | http://dx.doi.org/10.1080/21597081.2016.1157666 |
Status: | Published |
Publisher: | Taylor & Francis |
Identification Number: | 10.1080/21597081.2016.1157666 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:95995 |