Sharma, R, Deacon, SE, Nowak, D et al. (7 more authors) (2016) Label-free electrochemical impedance biosensor to detect human interleukin-8 in serum with sub-pg/ml sensitivity. Biosensors and Bioelectronics, 80. pp. 607-613. ISSN 0956-5663
Abstract
Biosensors with high sensitivity and short time-to-result that are capable of detecting biomarkers in body fluids such as serum are an important prerequisite for early diagnostics in modern healthcare provision. Here, we report the development of an electrochemical impedance-based sensor for the detection in serum of human interleukin-8 (IL-8), a pro-angiogenic chemokine implicated in a wide range of inflammatory diseases. The sensor employs a small and robust synthetic non-antibody capture protein based on a cystatin scaffold that displays high affinity for human IL-8 with a KD of 35±10 nM and excellent ligand specificity. The change in the phase of the electrochemical impedance from the serum baseline, ∆θ(ƒ), measured at 0.1 Hz, was used as the measure for quantifying IL-8 concentration in the fluid. Optimal sensor signal was observed after 15 min incubation, and the sensor exhibited a linear response versus logarithm of IL-8 concentration from 900 fg/ml to 900 ng/ml. A detection limit of around 90 fg/ml, which is significantly lower than the basal clinical levels of 5–10 pg/ml, was observed. Our results are significant for the development of point-of-care and early diagnostics where high sensitivity and short time-to-results are essential.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
Keywords: | Label-free biosensor; Antibody mimetic protein; Interleukin-8; CXCL8; Electrochemical impedance spectroscopy; Point-of-care diagnostics |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Electronic & Electrical Engineering (Leeds) > Pollard Institute (Leeds) |
Funding Information: | Funder Grant number Royal Society WM110032 |
Depositing User: | Symplectic Publications |
Date Deposited: | 04 Mar 2016 16:36 |
Last Modified: | 11 Apr 2018 15:36 |
Published Version: | http://dx.doi.org/10.1016/j.bios.2016.02.028 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.bios.2016.02.028 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:95561 |