Gonçalves, A.D., Alexander, C., Roberts, C.J. et al. (3 more authors) (2016) The effect of protein concentration on the viscosity of a recombinant albumin solution formulation. RSC Advances, 6. pp. 15143-15154.
Abstract
The effect of protein concentration on solution viscosity in a commercially available biopharmaceutical formulation of recombinant albumin (rAlbumin) was studied. The level of protein aggregation with concentration and its impact on solution viscosity was investigated. Theoretical models predicting viscosity with concentration were applied to these data, and a model that accounts for multiple protein species in solution provided the best fit. The results highlight the need to account for heterogeneity in the level of aggregation when addressing the increase of viscosity observed at high concentrations of protein solutions, a significant issue for the manufacture and use of protein-based therapeutics.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 Royal Society of Chemistry. This is an author produced version of a paper subsequently published in RSC Advances. Uploaded in accordance with the publisher's self-archiving policy. |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > Department of Chemistry (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 19 Feb 2016 14:20 |
Last Modified: | 06 Nov 2017 06:37 |
Published Version: | http://dx.doi.org/10.1039/C5RA21068B |
Status: | Published |
Publisher: | Royal Society of Chemistry |
Refereed: | Yes |
Identification Number: | 10.1039/C5RA21068B |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:95292 |