Cannon, DT, Liu, J, Sakurai, R et al. (2 more authors) (2016) Impaired lung mitochondrial respiration following perinatal nicotine exposure in rats. Lung, 194 (2). pp. 325-328. ISSN 0341-2040
Abstract
Perinatal smoke/nicotine exposure predisposes to chronic lung disease and morbidity. Mitochondrial abnormalities may contribute as the PPARγ pathway is involved in structural and functional airway deficits after perinatal nicotine exposure. We hypothesized perinatal nicotine exposure results in lung mitochondrial dysfunction that can be rescued by rosiglitazone (RGZ; PPARγ receptor agonist). Sprague-Dawley dams received placebo (CON), nicotine (NIC, 1 mg.kg-1), or NIC+RGZ (3 mg.kg-1) daily from embryonic day 6 to postnatal day 21. Parenchymal lung (~10mg) was taken from adult male offspring for mitochondrial assessment in situ. ADP-stimulated O2 consumption was less in NIC and NIC+RGZ compared to CON (F[2,14]=17.8; 4.5±0.8 and 4.1±1.4 vs. 8.8±2.5 pmol.s.mg-1; p<0.05). The respiratory control ratio for ADP, an index of mitochondrial coupling, was reduced in NIC and remediated in NIC+RGZ (F[2,14]=3.8; p<0.05). Reduced mitochondrial oxidative capacity and abnormal coupling was evident after perinatal nicotine exposure. Rosiglitazone improved mitochondrial function through tighter coupling of oxidative phosphorylation.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016, Springer. This is an author produced version of a paper published in Lung. Uploaded in accordance with the publisher's self-archiving policy. The final publication is available at Springer via http://dx.doi.org/10.1007/s00408-016-9859-2 |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 01 Mar 2016 14:48 |
Last Modified: | 21 Jul 2017 13:56 |
Published Version: | http://dx.doi.org/10.1007/s00408-016-9859-2 |
Status: | Published |
Publisher: | Springer Verlag |
Identification Number: | 10.1007/s00408-016-9859-2 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:95211 |