Cheng, F, Yin, L-L, Zhou, J et al. (5 more authors) (2016) Interactions between 2-Cys peroxiredoxins and ascorbate in autophagosome formation during the heat stress response in Solanum lycopersicum. Journal of Experimental Botany, 67 (6). pp. 1919-1933. ISSN 0022-0957
Abstract
2-Cys peroxiredoxins (2-CPs) function in the removal of hydrogen peroxide and lipid peroxides but their precise roles in the induction of autophagy (ATG) have not been characterized. Here we show that heat stress, which is known to induce oxidative stress, leads to the simultaneous accumulation of transcripts encoding 2-CPs and ATG proteins, and also autophagosomes in tomato plants. Virus-induced gene silencing of the tomato 2-CP1, 2-CP2 and 2-CP1/2 resulted in an increased sensitivity of tomato plants to heat stress. Silencing 2-CP2 or 2-CP1/2 increased the levels of transcripts associated with ascorbate biosynthesis but had no effects on the glutathione pool in the absence of stress. However, the heat-induced accumulation of transcripts associated with the water-water cycle was compromised by the loss of 2-CP1/2 functions. The transcript levels of autophagy-related genes ATG5 and ATG7 were higher in plants with impaired 2-CP1/2 functions, and the formation of autophagosomes was increased, together with an accumulation of oxidized and insoluble proteins. Silencing of ATG5 or ATG7 increased the levels of 2-CPs transcripts and protein but decreased heat stress tolerance. These results demonstrate that 2-CPs fulfill a pivotal role in heat stress tolerance in tomato, via interactions with ascorbate-dependent pathways and autophagy.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Editors: |
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Copyright, Publisher and Additional Information: | © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
Keywords: | antioxidant metabolism; autophagy; 2-Cys peroxiredoxin; heat tolerance; oxidized protein; Solanum lycopersicum; water-water cycle |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 08 Feb 2016 13:58 |
Last Modified: | 21 Nov 2018 13:38 |
Published Version: | http://dx.doi.org/10.1093/jxb/erw013 |
Status: | Published |
Publisher: | Oxford University Press |
Identification Number: | 10.1093/jxb/erw013 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:94704 |