Yorucu, C., Lau, K., Mittar, S. et al. (4 more authors) (2016) Raman spectroscopy detects melanoma and the tissue surrounding melanoma using tissue-engineered melanoma models. Applied Spectroscopy Reviews, 51 (4). pp. 243-257. ISSN 0570-4928
Abstract
Invasion of melanoma cells from the primary tumour involves interaction with adjacent tissues and extracellular matrix. The extent of this interaction is not fully understood. In this study Raman spectroscopy was applied to cryo-sections of established 3D models of melanoma in human skin. Principal component analysis was used to investigate differences between the tumour and normal tissue and between the peri-tumour area and the normal skin. Two human melanoma cells lines A375SM and C8161 were investigated and compared in 3D melanoma models. Changes were found in protein conformations and tryptophan configurations across the entire melanoma samples, in tyrosine orientation and in more fluid lipid packing only in tumour dense areas, and in increased glycogen content in the peri-tumour areas of melanoma. Raman spectroscopy revealed changes around the perimeter of a melanoma tumour as well as detecting differences between the tumour and the normal tissue.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 Ceyla Yorucu, Katherine Lau, Shweta Mittar, Nicola H. Green, Ahtasham Raza, Ihtesham Ur Rehman, and Sheila MacNeil. Published with license by Taylor & Francis. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Keywords: | Raman spectroscopy; melanoma; metastases; tissue engineering |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Materials Science and Engineering (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 22 Feb 2016 16:41 |
Last Modified: | 22 Feb 2016 16:41 |
Published Version: | http://dx.doi.org/10.1080/05704928.2015.1126840 |
Status: | Published |
Publisher: | Taylor & Francis |
Refereed: | Yes |
Identification Number: | 10.1080/05704928.2015.1126840 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:94033 |