Guo, Y orcid.org/0000-0003-4607-7356, Sakonsinsiri, C, Nehlmeier, I et al. (6 more authors) (2016) Compact, Polyvalent Mannose Quantum Dots as Sensitive, Ratiometric FRET Probes for Multivalent Protein–Ligand Interactions. Angewandte Chemie International Edition, 55 (15). pp. 4738-4742. ISSN 1433-7851
Abstract
A highly-efficient cap-exchange approach for preparing compact, dense polyvalent mannose-capped quantum dots (QDs) has been developed. The resulting QDs have been successfully used to probe multivalent interactions of HIV/Ebola receptors DC-SIGN and DC-SIGNR (collectively termed as DC-SIGN/R) via a sensitive, ratiometric Förster resonance energy transfer (FRET) assay. The QD probes specifically bind DC-SIGN, but not its closely related receptor DC-SIGNR, which is further confirmed by its specific blocking of DC-SIGN engagement with the Ebola virus glycoprotein. Tuning the QD surface mannose valency reveals that DC-SIGN binds more efficiently to densely packed mannosides and a FRET-based thermodynamic study reveals the binding is enthalpy driven. This work thus establishes QD-FRET as a rapid, sensitive technique for probing structure and thermodynamics of multivalent protein-ligand interactions.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
Keywords: | carbohydrates; FRET; proteins; quantum dots; viral inhibition |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) > Inorganic Chemistry (Leeds) The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) > Organic Chemistry (Leeds) The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) > FSN Chemistry and Biochemistry (Leeds) The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) |
Funding Information: | Funder Grant number Wellcome Trust 097354/Z/11/Z The Wellcome Trust UNSPECIFIED |
Depositing User: | Symplectic Publications |
Date Deposited: | 25 Jun 2019 09:28 |
Last Modified: | 23 Jun 2023 21:56 |
Status: | Published |
Publisher: | Wiley-VCH Verlag |
Identification Number: | 10.1002/anie.201600593 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:92858 |
Commentary/Response Threads
- Guo, Y, Sakonsinsiri, C, Nehlmeier, I, Fascione, MA, Zhang, H, Wang, W, Pöhlmann, S, Turnbull, WB and Zhou, D Compact, Polyvalent Mannose Quantum Dots as Sensitive, Ratiometric FRET Probes for Multivalent Protein–Ligand Interactions. (deposited 25 Jun 2019 09:28) [Currently Displayed]