Bulgakova, N.A. orcid.org/0000-0002-3780-8164, Grigoriev, I., Yap, A.S. et al. (2 more authors) (2013) Dynamic microtubules produce an asymmetric E-cadherin-Bazooka complex to maintain segment boundaries. Journal of Cell Biology, 201 (6). pp. 887-901. ISSN 0021-9525
Abstract
Distributing junctional components around the cell periphery is key for epithelial tissue morphogenesis and homeostasis. We discovered that positioning of dynamic microtubules controls the asymmetric accumulation of E-cadherin. Microtubules are oriented preferentially along the dorso-ventral axis in Drosophila melanogaster embryonic epidermal cells, and thus more frequently contact E-cadherin at dorso-ventral cell-cell borders. This inhibits RhoGEF2, reducing membrane recruitment of Rho-kinase, and increasing a specific E-cadherin pool that is mobile when assayed by fluorescence recovery after photobleaching. This mobile E-cadherin is complexed with Bazooka/Par-3, which in turn is required for normal levels of mobile E-cadherin. Mobile E-cadherin-Bazooka prevents formation of multicellular rosette structures and cell motility across the segment border in Drosophila embryos. Altogether, the combined action of dynamic microtubules and Rho signaling determines the level and asymmetric distribution of a mobile E-cadherin-Bazooka complex, which regulates cell behavior during the generation of a patterned epithelium.
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Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2013 Bulgakova et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
Keywords: | Animals; Animals, Genetically Modified; Cadherins; Cell Polarity; Drosophila Proteins; Drosophila melanogaster; Epithelial Cells; Epithelium; Female; Green Fluorescent Proteins; Intracellular Signaling Peptides and Proteins; Male; Membrane Proteins; Microtubules; Multiprotein Complexes; Signal Transduction; rho GTP-Binding Proteins |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 03 Aug 2016 14:20 |
Last Modified: | 23 Jun 2023 21:56 |
Published Version: | http://dx.doi.org/10.1083/jcb.201211159 |
Status: | Published |
Publisher: | Rockefeller University Press |
Refereed: | Yes |
Identification Number: | 10.1083/jcb.201211159 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:92785 |
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