Tahmassebi, J, Drogkari, E and Wood, SR (2015) A study of the control of oral plaque biofilms via antibacterial photodynamic therapy. European Archives of Paediatric Dentistry, 16 (6). 433 - 440. ISSN 1818-6300
Abstract
Aim: The aim of this study was to provide preliminary data on the most effective erythrosine concentration and light dose for the erythrosine-based photodynamic therapy (PDT) of oral plaque biofilms formed in vivo. Method: A randomized controlled study with 15 volunteers was carried out to investigate the effect of photosensitiser and light dose on the killing of bacteria in oral plaque biofilms formed in vivo. All volunteers wore a removable in situ appliance carrying 6 enamel slabs for two phases of two weeks each. During this time, plaque biofilms accumulated on the enamel slabs. The slabs were then removed from the appliances for PDT-treatment in vitro. In the first phase of the study, erythrosine doses of 22 and 220uM were used for the photodynamic treatment of the biofilms. In the second phase, the erythrosine concentration was kept constant, and the light dose was varied. Following treatment, the biofilms were disaggregated, and the total bacterial killing determined using colony counting. Results: The erythrosine dose of 220uM caused the most cell killing relative to controls. Fifteen minutes continuous irradiation with light and light fractionation of 5x1 minute irradiation separated by 2 minute dark recovery periods were found to be the most effective bactericidal regimes. Conclusion: Erythrosine-based PDT shows promise as an anti-bacterial treatment for oral plaque biofilms. Further research is needed to prove its clinical and cost-effectiveness compared with current best practice.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | (c) European Academy of Paediatric Dentistry 2015. This is an author produced version of a paper published in European Archives of Paediatric Dentistry. Uploaded in accordance with the publisher's self-archiving policy. The final publication is available at Springer via http://dx.doi.org/10.1007/s40368-014-0165-5 |
Keywords: | Plaque; Biofilms control; Photoactive solutions; Erythrosine; PDT |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Dentistry (Leeds) > Oral Biology (Leeds) The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Dentistry (Leeds) > Paediatric Dentistry (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 11 Dec 2015 14:01 |
Last Modified: | 05 Dec 2016 06:31 |
Published Version: | http://dx.doi.org/10.1007/s40368-014-0165-5 |
Status: | Published |
Publisher: | European Academy of Paediatric Dentistry |
Identification Number: | 10.1007/s40368-014-0165-5 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:92751 |