Chilton, CH, Crowther, GS, Śpiewak, K et al. (4 more authors) (2016) Potential of lactoferrin to prevent antibiotic-induced Clostridium difficile infection. Journal of Antimicrobial Chemotherapy, 71 (4). pp. 975-985. ISSN 0305-7453
Abstract
Objectives: C. difficile infection (CDI) is a global healthcare problem. Recent evidence suggests that the availability of iron may be important for C. difficile growth. This study evaluated the comparative effects of iron-depleted (1% Fe3+ saturated) bovine apo-lactoferrin (apo-bLf) and iron-saturated (85% Fe3+ saturated) bovine holo-lactoferrin (holo-bLf) in a human in vitro gut model that simulates CDI. Methods: Two parallel triple-stage chemostat gut models were inoculated with pooled human faeces and spiked with C. difficile spores (strain 027 210, PCR ribotype 027). Holo- or apo-bLf was instilled (5mg/mL, once daily) for 35 days. After 7 days, clindamycin was instilled (33.9mg/L, four times daily) to induce simulated CDI. Indigenous microflora populations, C. difficile total counts and spores , cytotoxin titres, short-chain fatty acids, biometals, lactoferrin, and iron content of lactoferrin were monitored daily. Results: In the apo-bLf model, germination of C. difficile spores occurred 6 days post-clindamycin, followed by rapid vegetative cell proliferation and detectable toxin. By contrast, in the holo-bLf model, only a modest vegetative cell population was observed until 16 days post-antibiotic. Notably, no toxin was detected in this model. In separate batch culture experiments, holo-bLf prevented C. difficile vegetative cell growth and toxin production, whereas apo-bLf and iron alone did not. Conclusions: Holo-bLf but not apo-bLf delayed C. difficile growth and prevented toxin production in a human gut model of CDI. This inhibitory effect may be iron-independent. These observations suggest that bLf in its iron-saturated state could be used as a novel preventative or treatment strategy for CDI.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © The Author 2016. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. This is an Open Access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0, which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
Keywords: | Iron, diarrhoea, colitis |
Dates: |
|
Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > Institute of Molecular Medicine (LIMM) (Leeds) > Section of Molecular Gastroenterology (Leeds) |
Funding Information: | Funder Grant number Academy of Medical Sciences RB4853 |
Depositing User: | Symplectic Publications |
Date Deposited: | 08 Dec 2015 12:30 |
Last Modified: | 23 Jun 2023 21:56 |
Published Version: | https://dx.doi.org/10.1093/jac/dkv452 |
Status: | Published |
Publisher: | Oxford University Press |
Identification Number: | 10.1093/jac/dkv452 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:92533 |