Fisher, M, Williamson, DJ, Burslem, GM et al. (11 more authors) (2015) Trivalent Gd-DOTA reagents for modification of proteins. RSC Advances, 5 (116). 96194 - 96200. ISSN 2046-2069
Abstract
The development of novel protein-targeted MRI contrast agents crucially depends on the ability to derivatise suitable targeting moieties with a high payload of relaxation enhancer (e.g., gadolinium(III) complexes such as Gd-DOTA), without losing affinity for the target proteins. Here, we report robust synthetic procedures for the preparation of trivalent Gd-DOTA reagents with various chemical handles for site-specific modification of biomolecules. The reagents were shown to successfully label proteins through isothiocyanate ligation or through site-specific thiol–maleimide ligation and strain-promoted azide–alkyne cycloaddition.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2015, Royal Society of Chemistry. This is an open access article licensed under a Creative Commons Attribution 3.0 Unported Licence. http://creativecommons.org/licenses/by/3.0/ |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) > Organic Chemistry (Leeds) The University of Leeds > Faculty of Medicine and Health (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 06 Nov 2015 11:20 |
Last Modified: | 12 Feb 2019 13:44 |
Published Version: | http://dx.doi.org/10.1039/C5RA20359G |
Status: | Published |
Publisher: | Royal Society of Chemistry |
Identification Number: | 10.1039/C5RA20359G |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:91538 |